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Image Search Results
Journal: Journal of Neuroscience
Article Title: Neonatal E. Coli Infection Causes Neuro-Behavioral Deficits Associated with Hypomyelination and Neuronal Sequestration of Iron
doi: 10.1523/jneurosci.0708-13.2013
Figure Lengend Snippet: Figure 4. Reduced number of oligodendrocytes in the corpus callosum and motor cortex of juvenile mice exposed to E. coli as neonates.Neonatalmice(P3)wereinjectedsubcutaneouslywitheithersaline(CON)orE.coli(ELI),andbraintissuefrommaleand female mice was collected at P35 and Olig2 (red) and GFAP (green) or Olig2 (red) and NG2 (green) labeling was determined. Representativeimagesof(A)Olig2,(B)Olig2/GFAP,(C)Olig2/NG2fromtheCCofCONandELImiceareshown.Averagenumberof Olig2 /NG2 cells in the (D) MCX, (E) CC, and (F) hippocampus (HPC). Average number of Olig2 /NG2 cells in the (G) MCX, (H)CC,and(I)HPC.BarsrepresentthemeanSEM(n6).Meanswithasteriskaresignificantlydifferentfromcontrols(p 0.05), and means with plus sign tend to be different from controls (p 0.1).
Article Snippet: To double label for Olig2 and GFAP, sections were first labeled for
Techniques: Labeling
Journal: Journal of Neuroscience
Article Title: Neonatal E. Coli Infection Causes Neuro-Behavioral Deficits Associated with Hypomyelination and Neuronal Sequestration of Iron
doi: 10.1523/jneurosci.0708-13.2013
Figure Lengend Snippet: Figure8. ELI-associatedincreaseinL-ferritindidnotcolocalizewithmicroglia,astrocytes,oroligodendrocytes.Neonatalmice (P3)wereinjectedsubcutaneouslywitheithersaline(CON)orE.coli(ELI),andbraintissuefrommaleandfemalemicewascollected at P12 and labeling for Iba-1 (pink), GFAP (white), Olig2 (light blue), and L-ferritin (L-Fer; red). Representative pictures of (A) microglia(Iba-1),(D)astrocytes(GFAP),and(G)oligodendrocytes(Olig2)inthecorpuscallosum(proximaltothehippocampalCA1 region)areshown.QuantificationofimmunopositivegliaintheCC,HP,andCXfor(B)Iba-1,(E)GFAP,and(H)Olig2areprovided. Representative images of colabeling with L-ferritin for (C) Iba-1, (F) GFAP, and (I) Olig2 are also provided. Bars represent the mean SEM (n 4). Means with asterisk are significantly different from controls (p 0.05). Means with plus sign denote a tendency (p 0.1). Scale bar, 100 M.
Article Snippet: To double label for Olig2 and GFAP, sections were first labeled for
Techniques: Labeling